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A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid.

Yu CL, Kale Y, Gopishetty S, Louie TM, Subramanian M

Center for Biocatalysis & Bioprocessing, The University of Iowa, Oakdale Research Park, 2501 Crosspark Road, Suite C100, Coralville, IA 52241, USA.

A unique heterotrimeric caffeine dehydrogenase was purified from Pseudomonas sp. strain CBB1. This enzyme oxidized caffeine to trimethyluric acid stoichiometrically and hydrolytically, without producing hydrogen peroxide. The enzyme was not NAD(P)(+) dependent; coenzyme Q(0) was the preferred electron acceptor. The enzyme was specific for caffeine and theobromine and showed no activity with xanthine.

Published 8 January 2008 in J Bacteriol, 190(2): 772-6.
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